The CRIPSR Cas9 Bacterial Genomic Edition Kit - unboxing

LB Agar provide a solid media nutrient source for bacteria to grow on

Heated Agar is dissolved in water, it will solidify when it cools

Agar plates (petri dish)

LB Agar + Streptomycin antibiotics

Finished plates stacked to cool off in space efficient pyramid

HME63 E.coli bacterial stab can keeps bacteria safe for long term storage in a fridge

Bacterial growth after 24 hours in 37C - normal agar plate left - agar+strep right with zero growth

NIce bacteria growth after 24 hours

All bacteria died on the right plate with antibiotics

Pipeting bacterial transsformation mix - 10% Polyethylene Glocol (PEG) 3350 - 25mM CaCl2

Competent transformation mix - bacteria are able to intake foreing DNA

Pipeting Cas9 + gRNA + template DNA - 10uL each - to the competent mix

Pipeting Cas9 + gRNA + template DNA - 10uL each

Cas9 + gRNA + template DNA + transformation mix

Tranformation mix + LB agar media - now will bacteria recover and replicate the DNA and perform the CRISPR engineering process

Using inoculation loop to gently spread the bacteria on the Agar+Strept plates

Pipeting engineered bacteria on second Agar + Strep plate

White dots are CRISPR edited bacteria growing on Strep Agar plate